Basic Concepts in Clinical Biochemistry: A Practical Guide by Vijay Kumar & Kiran Dip Gill

Author:Vijay Kumar & Kiran Dip Gill
Language: eng
Format: epub
Publisher: Springer Singapore, Singapore

Working uric acid standard (μl)

–

50

100

150

200

250

500

–

–

Uric acid amount (μg)

--

10

20

30

40

50

100

–

–

Protein-free filtrate of serum/urine (ml)

–

–

–

–

–

–

–

3

3

Distilled water (ml)

3

2.95

2.90

2.85

2.80

2.75

2.5

–

–

Na2CO3 (ml)

1

1

1

1

1

1

1

1

1

Phosphotungstic acid (ml)

1

1

1

1

1

1

1

1

1

Mix well and keep for 20 min at room temperature, read absorbance at 700 nm

20.6 Calculations

Plot a graph between amount of uric acid at x-axis and absorbance at y-axis and extrapolate uric acid concentration of given sample from the graph. The uric acid concentration can also be calculated by using equation:

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